Proteins and free aminoacids from microalgae suitable for gas depuration

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Callejo   The use of microalgae in integrated gas purification processes has taken a special interest in recent years, mainly due to its ability to capture CO2. These applications involve the generation of biomass that can be used to obtain bioproducts and/or bioenergy.

   However, the development of these processes requires an improvement in the cell rupture and biocompounds extraction methods. The aim of this study was to evaluate a method for obtaining proteins and free amino acids (FAN) from Chlorella vulgaris.

J.A. Callejo*, M. Ramírez, J. Bolívar, A. Valle, D. Cantero

Departamento de Ingeniería Química y Tecnología de los Alimentos. Facultad de Ciencias. Universidad de Cádiz. Instituto Universitario de Investigación Vitivinícola y Agroalimentario (IVAGRO). Av. República Saharaui S/N. 11510 Puerto Real, Spain.

   Competing interests: The author has declared that no competing interests exist.

   Academic editor: Carlos N Díaz.

   Content quality: This paper has been peer reviewed by at least two reviewers. See scientific committee here

   Citation: J.A. Callejo, M. Ramírez, J. Bolívar, A. Valle, D. Cantero, 2017, Proteins and free aminoacids from microalgae suitable for gases depuration, IV International Conference of Odours and VOCs in the Environment, Valladolid, Spain, www.olores.org.

   Copyright: 2018 Olores.org. Open Content Creative Commons license. It is allowed to download, reuse, reprint, modify, distribute, and/or copy articles in olores.org website, as long as the original authors and source are cited. No permission is required from the authors or the publishers.

   ISBN: 978-84-697-7359-8

   Keyword: Gases depuration with Chlorella vulgaris, Cell wall lysis, Autolysis, Protein hydrolysis, Proteins and free-amino-acids extracts

 

Abstract

   The use of microalgae in integrated gas purification processes has taken a special interest in recent years, mainly due to its ability to capture CO2. These applications involve the generation of biomass that can be used to obtain bioproducts and/or bioenergy.

   However, the development of these processes requires an improvement in the cell rupture and biocompounds extraction methods. The aim of this study was to evaluate a method for obtaining proteins and free amino acids (FAN) from Chlorella vulgaris.

    For this purpose, fresh biomass was used as the starting material and, in order to establish a methodology, two sequential phases were carried out: i) a cell membrane rupture to release the proteins, by an enzymatic treatment with cellulase at 50°C and pH 5, and ii) an autolysis of the proteins extracted to FAN, by keeping the extract at a temperature of 50°C for 24 h and several pH values. For the optimization of the method, a statistical design of response surface (composite central design) was used.

   The variables evaluated were the time of the enzymatic reaction with cellulase, and the pH value of the heat treatment. The response variables to maximize were the yield of proteins, FAN, and both together. The optimal values obtained for protein extraction were, 3.5 h and pH 8.5; 2.9 h and pH 8.2 for FAN extraction; and for both, 3.3 h and pH 8.3. In the model validation, under the latter conditions, yields of proteins and FAN of 14,2% and 0,31% on dry biomass, respectively, were obtained.

 

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